請輸入網站標題
取消
清空記錄
歷史記錄
清空記錄
歷史記錄
瀏覽器自帶分享功能也很好用哦~試劑盒特點：
1. 樣本起始量10pg-100ng DNA；
2. 無接頭二聚體污染，無需接頭稀釋；
3. 單端有96個不同index，雙端有96個dual-index，能夠避免index hopping；
4. 適用于CHIP-seq，cfDNA, 咽拭子及肛拭子樣本等提取的微量DNA。
1. 樣本起始量10pg-100ng DNA；
2. 無接頭二聚體污染，無需接頭稀釋；
3. 單端有96個不同index，雙端有96個dual-index，能夠避免index hopping；
4. 適用于CHIP-seq，cfDNA, 咽拭子及肛拭子樣本等提取的微量DNA。
在線客服
客服一
產品介紹
|
貨號 |
品名 |
規格 |
|
0344-32 |
Ovation® Ultralow System V2 |
32 |
|
0344NB-08 |
Ovation® Ultralow System V2 |
8 |
|
0344NB-A01 |
Ovation® Ultralow System V2 |
A01 |
|
0344NB-32 |
Ovation® Ultralow System V2 |
32 |
|
9149-A01 |
Ovation® Ultralow System V2 + Dual Unique Index + Molecular Tag |
A01 |
|
9123-A01 |
Ovation® Ultralow V2 KarisDx |
A01 |
|
0344-32 |
Ovation® Ultralow System V2 |
32 |
試劑盒特點：
1. 樣本起始量10pg-100ng DNA；
2. 無接頭二聚體污染，無需接頭稀釋；
3. 單端有96個不同index，雙端有96個dual-index，能夠避免index hopping；
4. 適用于CHIP-seq，cfDNA, 咽拭子及肛拭子樣本等提取的微量DNA。
試劑盒文獻：
1. c-Maf-dependent regulatory T cells mediate immunological tolerance to intestinal microbiota
22 April, 2017
Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Galan C, Bonneau R, Littman DR
Both microbial and host genetic factors contribute to the pathogenesis of autoimmune disease1-4. Accumulating evidence suggests that microbial species that potentiate chronic inflammation, as in inflammatory bowel disease (IBD), often also colonize healthy individuals. These microbes, including the Helicobacter species, have the propensity to induce autoreactive T cells and are collectively referred to as pathobionts4-8. However, an understanding of how such T cells are constrained in healthy individuals is lacking. Here we report that host tolerance to a potentially pathogenic bacterium, Helicobacter hepaticus (H. hepaticus), is mediated by induction of RORγt+Foxp3+ regulatory T cells (iTreg) that selectively restrain pro-inflammatory TH17 cells and whose function is dependent on the transcription factor c-Maf. Whereas H. hepaticus colonization of wild-type mice promoted differentiation of RORγt-expressing microbe-specific iTreg in the large intestine, in disease-susceptible IL-10-deficient animals there was instead expansion of colitogenic TH17 cells. Inactivation of c-Maf in the Treg compartment likewise impaired differentiation of bacteria-specific iTreg, resulting in accumulation of H. hepaticus-specific inflammatory TH17 cells and spontaneous colitis. In contrast, RORγt inactivation in Treg only had a minor effect on bacterial-specific Treg-TH17 balance, and did not result in inflammation. Our results suggest that pathobiont-dependent IBD is a consequence of microbiota-reactive T cells that have escaped this c-Maf-dependent mechanism of iTreg-TH17 homeostasis.
doi: https://doi.org/10.1101/129601
2. Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin
21 February, 2017
The yak is remarkable for its adaptation to high altitude and occupies a central place in the economies of the mountainous regions of Asia. At lower elevations, it is common to hybridize yaks with cattle to combine the yak's hardiness with the productivity of cattle. Hybrid males are sterile, however, preventing the establishment of stable hybrid populations, but not a limited introgression after backcrossing several generations of female hybrids to male yaks. Here we inferred bovine haplotypes in the genomes of 76 Mongolian yaks using high-density SNP genotyping and whole-genome sequencing. These yaks inherited ～1.3% of their genome from bovine ancestors after nearly continuous admixture over at least the last 1,500 years. The introgressed regions are enriched in genes involved in nervous system development and function, and particularly in glutamate metabolism and neurotransmission. We also identified a novel mutation associated with a polled (hornless) phenotype originating from Mongolian Turano cattle. Our results suggest that introgressive hybridization contributed to the improvement of yak management and breeding.
doi: 10.1016/j.molcel.2017.01.026
產品介紹
|
貨號 |
品名 |
規格 |
|
0344-32 |
Ovation® Ultralow System V2 |
32 |
|
0344NB-08 |
Ovation® Ultralow System V2 |
8 |
|
0344NB-A01 |
Ovation® Ultralow System V2 |
A01 |
|
0344NB-32 |
Ovation® Ultralow System V2 |
32 |
|
9149-A01 |
Ovation® Ultralow System V2 + Dual Unique Index + Molecular Tag |
A01 |
|
9123-A01 |
Ovation® Ultralow V2 KarisDx |
A01 |
|
0344-32 |
Ovation® Ultralow System V2 |
32 |
試劑盒特點：
1. 樣本起始量10pg-100ng DNA；
2. 無接頭二聚體污染，無需接頭稀釋；
3. 單端有96個不同index，雙端有96個dual-index，能夠避免index hopping；
4. 適用于CHIP-seq，cfDNA, 咽拭子及肛拭子樣本等提取的微量DNA。
試劑盒文獻：
1. c-Maf-dependent regulatory T cells mediate immunological tolerance to intestinal microbiota
22 April, 2017
Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Galan C, Bonneau R, Littman DR
Both microbial and host genetic factors contribute to the pathogenesis of autoimmune disease1-4. Accumulating evidence suggests that microbial species that potentiate chronic inflammation, as in inflammatory bowel disease (IBD), often also colonize healthy individuals. These microbes, including the Helicobacter species, have the propensity to induce autoreactive T cells and are collectively referred to as pathobionts4-8. However, an understanding of how such T cells are constrained in healthy individuals is lacking. Here we report that host tolerance to a potentially pathogenic bacterium, Helicobacter hepaticus (H. hepaticus), is mediated by induction of RORγt+Foxp3+ regulatory T cells (iTreg) that selectively restrain pro-inflammatory TH17 cells and whose function is dependent on the transcription factor c-Maf. Whereas H. hepaticus colonization of wild-type mice promoted differentiation of RORγt-expressing microbe-specific iTreg in the large intestine, in disease-susceptible IL-10-deficient animals there was instead expansion of colitogenic TH17 cells. Inactivation of c-Maf in the Treg compartment likewise impaired differentiation of bacteria-specific iTreg, resulting in accumulation of H. hepaticus-specific inflammatory TH17 cells and spontaneous colitis. In contrast, RORγt inactivation in Treg only had a minor effect on bacterial-specific Treg-TH17 balance, and did not result in inflammation. Our results suggest that pathobiont-dependent IBD is a consequence of microbiota-reactive T cells that have escaped this c-Maf-dependent mechanism of iTreg-TH17 homeostasis.
doi: https://doi.org/10.1101/129601
2. Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin
21 February, 2017
The yak is remarkable for its adaptation to high altitude and occupies a central place in the economies of the mountainous regions of Asia. At lower elevations, it is common to hybridize yaks with cattle to combine the yak's hardiness with the productivity of cattle. Hybrid males are sterile, however, preventing the establishment of stable hybrid populations, but not a limited introgression after backcrossing several generations of female hybrids to male yaks. Here we inferred bovine haplotypes in the genomes of 76 Mongolian yaks using high-density SNP genotyping and whole-genome sequencing. These yaks inherited ～1.3% of their genome from bovine ancestors after nearly continuous admixture over at least the last 1,500 years. The introgressed regions are enriched in genes involved in nervous system development and function, and particularly in glutamate metabolism and neurotransmission. We also identified a novel mutation associated with a polled (hornless) phenotype originating from Mongolian Turano cattle. Our results suggest that introgressive hybridization contributed to the improvement of yak management and breeding.
doi: 10.1016/j.molcel.2017.01.026
相關產品
滬ICP備2021005022號-1
版權所有?上海博競生物科技有限公司
本站使用T云建站搭建
品牌認證
1449天
已認證
品牌認證
1449天
已認證
選擇區號